Prentice Hall

Engineering



Bioprocess Engineering: Basic Concepts, 2/E
Michael L. Shuler, Cornell University
Fikret Kargi, Dokuz Eylul University

ISBN-10: 0130819085
ISBN-13: 9780130819086

Publisher: Prentice Hall
Copyright: 2002
Format: Cloth; 576 pp
Published: 10/31/2001

Suggested retail price: $137.00
Buy from myPearsonStore



For Senior-level and graduate courses in Biochemical Engineering, and for programs in Agricultural and Biological Engineering or Bioengineering.

This concise yet comprehensive text introduces the essential concepts of bioprocessing—internal structure and functions of different types of microorganisms, major metabolic pathways, enzymes, microbial genetics, kinetics and stoichiometry of growth and product information—to traditional chemical engineers and those in related disciplines. It explores the engineering principles necessary for bioprocess synthesis and design, and illustrates the application of these principles to modern biotechnology for production of pharmaceuticals and biologics, solution of environmental problems, production of commodities, and medical applications.

  • NEW - Concepts of validation and Good Manufacturing Practice (GMP) are introduced.

    • Helps students to better understand regulatory constraints on bioprocess development. Ex.___

  • NEW - Updated coverage of concepts.

    • Shows students the connection between traditional ideas and emerging areas—such as tissue engineering and gene therapy. Ex.___

  • NEW - Material on functional genomics and cellular engineering.

    • Provides students with new developments in biology as they impact bioprocess engineering. Ex.___

  • NEW - Expanded discussion of modeling approach.

    • Presents students with a clarified section on models in continuous cultures and adds cybernetic modeling. Ex.___

  • NEW - Expanded coverage of chromatography.

    • Introduces students to discussions of IMAC (immobilized metal affinity chromatography), use of fusion proteins, and porous supports. Ex.___

  • NEW - Expanded sections on metabolic engineering, animal cell culture, and protein processing.

    • Offers students information on analysis of metabolic pathways, bioreactor considerations for animal cells, and includes some recent examples. Ex.___

  • NEW - Additional examples and homework problems—e.g., on topics such as enzyme reaction; reactor operation and scale-up; purification; waste treatment; and genetically engineered cells.

    • Enables students to more thoroughly test their understanding of applied concepts. Ex.___

  • NEW - Reorganized coverage.

    • Gives students a more logical presentation of genetic instability, strategies for genetic engineering, and then an approach to selection of host expression system for production of a heterologous protein. Ex.___

  • Emphasis on novel bioprocessing technologies.

    • Provides students with discussions on metabolic pathways and regulation, bioreactors, and separation processes. Ex.___

  • Coverage on production of proteins from recombinant DNA technology.

    • Allows students to critically compare and evaluate the various techniques involved. Ex.___

  • Applications—To special systems and the particular characteristics of mixed cultures; genetically engineered cells; and plant and animal cells.

    • Reinforces the previously covered engineering and biological concepts while providing more detailed information about important new biological systems. Ex.___

  • Chapter-end suggested readings.

    • Encourages students to obtain a more in-depth understanding of key biological and engineering concepts. Ex.___

  • Concepts of validation and Good Manufacturing Practice (GMP) are introduced.

    • Helps students to better understand regulatory constraints on bioprocess development. Ex.___

  • Updated coverage of concepts.

    • Shows students the connection between traditional ideas and emerging areas—such as tissue engineering and gene therapy. Ex.___

  • Material on functional genomics and cellular engineering.

    • Provides students with new developments in biology as they impact bioprocess engineering. Ex.___

  • Expanded discussion of modeling approach.

    • Presents students with a clarified section on models in continuous cultures and adds cybernetic modeling. Ex.___

  • Expanded coverage of chromatography.

    • Introduces students to discussions of IMAC (immobilized metal affinity chromatography), use of fusion proteins, and porous supports. Ex.___

  • Expanded sections on metabolic engineering, animal cell culture, and protein processing.

    • Offers students information on analysis of metabolic pathways, bioreactor considerations for animal cells, and includes some recent examples. Ex.___

  • Additional examples and homework problems—e.g., on topics such as enzyme reaction; reactor operation and scale-up; purification; waste treatment; and genetically engineered cells.

    • Enables students to more thoroughly test their understanding of applied concepts. Ex.___

  • Reorganized coverage.

    • Gives students a more logical presentation of genetic instability, strategies for genetic engineering, and then an approach to selection of host expression system for production of a heterologous protein. Ex.___



Preface to the Second Edition.


Preface to the First Edition.

I. INTRODUCTION.

1. What is a Bioprocess Engineer?

Introductory Remarks. Biotechnology and Bioprocess Engineering. Biologists and Engineers Differ in Their Approach to Research. The Story of Penicillin: How Biologists and Engineers Work Together. Bioprocesses: Regulatory Constraints. Suggestions for Further Reading. Problems.

II. THE BASICS OF BIOLOGY: AN ENGINEER'S PERSPECTIVE.

2. An Overview of Biological Basics.

Are All Cells the Same? Cell Construction. Cell Nutrients. Summary. Suggestions for Further Reading. Problems.

3. Enzymes.

Introduction. How Enzymes Work. Enzyme Kinetics. Immobilized Enzyme Systems. Large-scale Production of Enzymes. Medical and Industrial Utilization of Enzymes. Summary. Suggestions for Further Reading. Problems.

4. How Cells Work.

Introduction. The Central Dogma. DNA Replication: Preserving and Propagating the Cellular Message. Transcription: Sending the Message. Translation: Message to Product. Metabolic Regulation. How the Cell Senses Its Extracellular Environment. Summary. Appendix: Examples of Regulation of Complex Pathways. Suggestions for Further Reading. Problems.

5. Major Metabolic Pathways.

Introduction. Bioenergetics. Glucose Metabolism: Glycolysis and the TCA Cycle. Respiration. Control Sites in Aerobic Glucose Metabolism. Metabolism of Nitrogenous Compounds. Nitrogen Fixation. Metabolism of Hydrocarbons. Overview of Biosynthesis. Overview of Anaerobic Metabolism. Overview of Autotrophic Metabolism. Summary. Suggestions for Further Reading. Problems.

6. How Cells Grow.

Introduction. Batch Growth. Quantifying Growth Kinetics. How Cells Grow in Continuous Culture. Summary. Suggestions for Further Reading. Problems.

7. Stoichiometry of Microbial Growth and Product Formation.

Introduction. Some Other Definitions. Stoichiometric Calculations. Theoretical Predictions of Yield Coefficients. Summary. Suggestions for Further Reading. Problems.

8. How Cellular Information is Altered.

Introduction. Evolving Desirable Biochemical Activities through Mutation and Selection. Natural Mechanisms for Gene Transfer and Rearrangement. Genetically Engineering Cells. Genomics. Summary. Suggestions for Further Reading. Problems.

III. ENGINEERING PRINCIPLES FOR BIOPROCESSES.

9. Operating Considerations for Bioreactors for Suspension and Immobilized Cultures.

Introduction. Choosing the Cultivation Method. Modifying Batch and Continuous Reactors. Immobolized Cell Systems. Solid-state Fermentations. Summary. Suggestions for Further Reading. Problems.

10. Selection, Scale-Up, Operation, and Control of Bioreactors.

Introduction. Scale-up and Its Difficulties. Bioreactor Instrumentation and Control. Sterilization of Process Fluids. Summary. Suggestions for Further Reading. Problems.

11. Recovery and Purification of Products.

Strategies to Recover and Purify Products. Separation of Insoluble Products. Cell Disruption. Separation of Soluble Products. Finishing Steps for Purification. Integration of Reaction and Separation. Summary. Suggestions for Further Reading. Problems.

IV. APPLICATIONS TO NONCONVENTIONAL BIOLOGICAL SYSTEMS.

12. Bioprocess Considerations in Using Animal Cell Cultures.

Structure and Biochemistry of Animal Cells. Methods Used for the Cultivation of Animal Cells. Bioreactor Considerations for Animal Cell Culture. Products of Animal Cell Cultures. Summary. Suggestions for Further Reading. Problems.

13. Bioprocess Considerations in Using Plant Cell Cultures.

Why Plant Cell Cultures? Plant Cells in Culture Compared to Microbes. Bioreactor Considerations. Economics of Plant Cell Tissue Cultures. Summary. Suggestions for Further Reading. Problems.

14. Utilizing Genetically Engineered Organisms.

Introduction. How the Product Influences Process Decisions. Guidelines for Choosing Host-Vector Systems. Process Constraints: Genetic Instability. Considerations in Plasmid Design to Avoid Process Problems. Predicting HostÐVector Interactions and Genetic Instability. Regulatory Constraints on Genetic Processes. Metabolic Engineering. Protein Engineering. Summary. Suggestions for Further Reading. Problems.

15. Medical Applications of Bioprocess Engineering.

Introduction. Tissue Engineering. Gene Therapy Using Viral Vectors. Bioreactors. Summary. Suggestions for Further Reading. Problems.

16. Mixed Cultures.

Introduction. Major Classes of Interactions in Mixed Cultures. Simple Models Describing Mixed-culture Interactions. Mixed Cultures in Nature. Industrial Utilization of Mixed Cultures. Biological Waste Treatment: An Example of the Industrial Utilization of Mixed Cultures. Summary. Suggestions for Further Reading. Problems.

17. Epilogue.

Appendix: Traditional Industrial Bioprocesses.

Anaerobic Bioprocesses. Aerobic Processes.

Suggestions for Further Reading.

Index.

DR. MICHAEL L. SHULER is Professor in the School of Chemical Engineering, Cornell University. His areas of research include structured models, heterologous protein expression systems, cell culture analogs for pharmacokinetic models, in-vitro toxicology, plant-cell tissue culture, microbial functional genomics, and bioremediation.

DR. FIKRET KARGI is Professor of Environmental Engineering at Dokuz Eylul University in Ismir, Turkey. His current research includes bioprocessing of wastes for production of commercial products, development of novel technologies for biological treatment of problematic wastewaters, nutrient removal, and novel biofilm reactor development.

The complete, fully updated introduction to biochemical and bioprocess engineering.

Bioprocess Engineering, Second Edition is a comprehensive update of the world's leading introductory textbook on biochemical and bioprocess engineering. Drs. Michael L. Shuler and Fikret Kargi review the relevant fundamentals of biochemistry, microbiology, and molecular biology, introducing key principles that enable bioprocess engineers to achieve consistent control over biological activity. This edition reflects powerful advances that are transforming the field, ranging from genetic sequencing to new techniques for producing proteins from recombinant DNA. It introduces techniques with broad application to the production of pharmaceuticals, biologics, and commodities; to medical applications such as tissue engineering and gene therapy; and for solving critical environmental problems. This new edition includes:

  • Essential biological basics: microorganism structure and functions, major metabolic pathways, enzymes, microbial genetics, kinetics, and stoichiometry of growth
  • New coverage of posttranslational processing of proteins-an essential technique for manufacturing therapeutic proteins
  • In-depth coverage of animal cell culture processes
  • New coverage of noncarbohydrate metabolism
  • Functional genomics and cellular engineering: concepts, techniques, and applications
  • Applying bioprocess engineering approaches to biomedical applications
  • Nonconventional biological systems applications, including host-vector systems for producing proteins from recombinant DNA
  • Extensive coverage of mixed cultures, including advanced wastewater treatment processes
  • Expanded coverage of modeling, including models in continuous cultures and cybernetic modeling
  • How the rapidly evolving governmental regulatory environment constrains bioprocess design and modification

Bioprocess Engineering, Second Edition makes extensive use of illustrations, examples, and problems, and contains extensive references for further reading as well as a detailed appendix describing traditional bioprocesses.

View a Sample Chapter PDF: /samplechapter/0130819085.pdf

  • Solutions Manual, 2/E
    Shuler & Kargi
    © 2002 | Prentice Hall | Paper | Estimated Availability: 02/19/2002
    ISBN-10: 0130895377 | ISBN-13: 9780130895370


Pearson Higher Education offers special pricing when you choose to package your text with other student resources. If you're interested in creating a cost-saving package for your students, contact your Pearson Higher Education representative for pricing and ordering information.

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