Basic Laboratory Methods for Biotechnology
Lisa A. Seidman, Madison Area Technical College
Cynthia J. Moore, Illinois State University

ISBN-10: 0137955359
ISBN-13: 9780137955350

Publisher: Benjamin Cummings
Copyright: 2000
Format: Spiral Bound; 751 pp
Published: 01/19/2000

Suggested retail price: $78.60
Not available for purchase at this time.

A primary text for courses that focus on basic laboratory methods in biotechnology, and an ancillary text for any biology course that includes a laboratory component that want to focus on biotechnology.

Unique in both content and approach, this text/reference provides a systematic introduction to basic laboratory methods from the perspective of the everyday work in the labs of the biotechnology/bioscience industry. Written at a level that is accessible to beginning college students with average math and science backgrounds. Provides the depth and detail useful to professional laboratory workers. It blends the background and theory provided by introductory chemistry texts with the practical concerns discussed in advanced lab manuals. It focuses on basic laboratory methods, the principles that underlie those methods, and the equipment that makes laboratory work possible.

  • Focuses on basic laboratory topics and methods that are fundamental to everyday work in all biotechnology/bioscience laboratories.

    • Makes the relationship between theory and practice explicit so that bench workers can immediately apply the information to their work in the laboratory. Ex.___

  • Consistent with the National Voluntary Skill Standards in Agricultural Biotechnology and the Biosciences.
  • Reflects the perspective of the biotechnology/biosciences industry.
  • Draws content from chemistry texts, molecular biology manuals, manufacturer and supplier information, and the useful laboratory lore which is often unwritten.
  • Emphasizes the exciting advances in biotechnology—Reflects the complexity and diversity of the roles, responsibilities, and skills associated with biotechnology/bioscience bench scientists and technicians particularly the key role they play in producing scientific knowledge and its practical applications.
  • Incorporates explicitly and implicitly throughout the principles behind good laboratory practices— Showing students why good practices are essential and how these practices lead to quality results and products.
  • Emphasizes that individuals employed in the bioscience industries need to be comfortable in an environment tightly regulated by industrial regulations and standards.
  • Emphasizes that standards and regulations embody what has long been taught to academic scientists as good laboratory technique, e.g., the importance of documentation such as SOP.
  • Introduces math early as a tool.

    • Explains math concepts in simple language, integrates math applications into many of the discussions, and provides a number of problems to help students apply math techniques. Ex.___

  • Provides a number of solved problems throughout.
  • Intersperses case studies, anecdotes, and familiar examples of techniques and applications throughout.
  • Contains worked examples throughout that demonstrate how to solve particular classes of problems or apply concepts.
  • How to perform calculations, how to use equations, and how to apply mathematical tools to laboratory situations are all included.
  • Provides Problem Sets for each topic or chapter.
  • Includes “practice problems” which have specific answers and discussion questions which are based on laboratory “scenarios.”
  • Features problems that: encourage critical thinking and application of concepts; develop judgment and laboratory “sense,” develop practical skills—Such as performing calculations, finding information in technical resources, using catalogues and product information, making educated decisions, following standard laboratory procedures, writing standard laboratory procedures, paying attention to the details which impact quality.
  • Features boxes that outline the specific steps in a procedure, and tables containing practical tips.
  • Includes a glossary and a comprehensive index—Allowing lab workers to quickly locate a procedure of interest.

I. INTRODUCTION TO THE BIOTECHNOLOGY WORKPLACE.

 1. Biotechnology and the Workplace.

 2. Introduction to a Safe Workplace.

 3. Biotechnology and the Regulation of Medical and Food Products.

II. PRODUCT QUALITY AND BIOTECHNOLOGY.

 4. Introduction to Product Quality Systems.

 5. Documentation: The Foundation of Quality.

 6. Quality Systems in the Laboratory.

 7. Quality Systems in the Production Facility.

III. MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW.

 8. Basic Math Techniques.

 9. Proportional Relationships.

10. Relationships and Graphing.

IV. DATA IN THE LABORATORY.

11. Descriptions of Data.

12. The Collection of Meaningful Data; Avoiding Errors in the Laboratory.

V. LABORATORY MEASUREMENTS.

13. Introduction to Quality Laboratory Measurements, Tests, and Assays.

14. Introduction to Instrumental Methods and Electricity.

15. The Measurement of Weight.

16. The Measurement of Volume.

17. The Measurement of Temperature.

18. The Measurement of pH, Selected Ions, and Conductivity.

19. Measurements Involving Light A: Basic Principles and Instrumentation.

20. Measurements Involving Light B: Applications and Methods.

VI. LABORATORY SOLUTIONS.

21. Preparation of Laboratory Solutions A: Concentration Expressions and Calculations.

22. Preparation of Laboratory Solutions B: Basic Procedures and Practical Information.

23. Laboratory Solutions to Support the Activity of Biological Macromolecules and Intact Cells.

24. Solutions: Associated Procedures and Information.

VII. BASIC SEPARATION METHODS.

25. Introduction to Filtration.

26. Introduction to Centrifugation.

27. Introduction to Bioseparations.

VIII. SAFETY IN THE LABORATORY.

28. Working Safely in the Laboratory: General Considerations and Physical Hazards.

29. Working Safely with Chemicals.

30. Working Safely with Biological Materials.

IX. COMPUTERS IN THE LABORATORY.

31. Computers: An Overview.

32. Data Handling with Computers.

33. Telecommunications and the Internet.

    For laboratory courses in Biotechnology.

     

    Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry’s oral tradition.

     

    The Second Edition features two new chapters covering Quality Assays and Tests (Chapters 24 and 25) and a new chapter on Culture Media for Intact Cells (Chapter 30). The authors have made revisions to update the safety chapters, the methods chapters, and have reorganized the units so that the information is presented in an order that mimics a typical class. They have also completely rewritten the Computers and Biotechnology chapters as well as the introductory chapter to more accurately reflect the evolving nature of the biotechnology industry. This new edition also reflects the changes in the field of biotechnology, how profit and other metrics affect research, the pursuit of new ideas, and the implementation of product testing and government approval.

     

  • 0321570146Basic Laboratory Methods for Biotechnology, 2/E
    Seidman & Moore
    © 2009 | Benjamin Cummings | Spiral Bound; 450 pages | Instock
    ISBN-10: 0321570146 | ISBN-13: 9780321570147
    Brief Description | Buy from myPearsonStore

"The authors have done an outstanding job of capturing the essential skills and applied theories of mathematics, physics, biology and chemistry that are pertinent to the training needs of workers in biotechnology. The information contained in these chapters represent a wealth of basic, practical knowledge that previously was not readily available in print, but more likely was acquired 'on the job'," — Dr. Gail Baughman, MiraCosta College

"The texts we have found for our biotechnology theory course are either too deep (molecular biology) or too shall (gee whiz). It is ironic that all three of the internships I have done in biotech companies have asked for the kinds of skills found in this text, but no other text seems to be available like it. I think that the book will be a best seller." — Bill Thieman, Ventura College

"The use of many worked out examples make this test especially strong as a reference for the technician." — David B. Shaw, Madison Area Technical College

LISA SEIDMAN received her Ph.D. from the University of Wisconsin and has taught for over twelve years in the Biotechnology Laboratory Technician Program at Madison Area Technical College, Madison, Wisconsin.

CYNTHIA MOORE received her doctoral degree from Temple University School of Medicine and has a career both in research and as a science educator. She is currently a faculty member in the Department of Biological Sciences at Illinois State University, Normal, Illinois.

The authors welcome comments and feedback from readers. You can e-mail Lisa Seidman at lseidman@madison.tec.wi.us and Cynthia Moore at cjmoor1@ilstu.edu.

This manual provides a systematic and very practical introduction to the bioscience laboratory. It will serve both as a primary text for undergraduate students preparing for a career in biotechnology and as a reference for laboratory professionals. It includes coverage of:

  • Math in the laboratory
  • Preparing solutions
  • Measurements
  • Safety
  • Separations
  • Using computers in the lab
  • Quality control
  • Data analysis

View a Sample Chapter PDF:

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