Basic Laboratory Methods for Biotechnology, 2/E
Lisa A. Seidman, Madison Area Technical College
Cynthia J. Moore, Illinois State University

ISBN-10: 0321570146
ISBN-13: 9780321570147

Publisher: Benjamin Cummings
Copyright: 2009
Format: Spiral Bound; 450 pp
Published: 10/29/2008

Suggested retail price: $77.33
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For laboratory courses in Biotechnology.

 

Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry’s oral tradition.

 

The Second Edition features two new chapters covering Quality Assays and Tests (Chapters 24 and 25) and a new chapter on Culture Media for Intact Cells (Chapter 30). The authors have made revisions to update the safety chapters, the methods chapters, and have reorganized the units so that the information is presented in an order that mimics a typical class. They have also completely rewritten the Computers and Biotechnology chapters as well as the introductory chapter to more accurately reflect the evolving nature of the biotechnology industry. This new edition also reflects the changes in the field of biotechnology, how profit and other metrics affect research, the pursuit of new ideas, and the implementation of product testing and government approval.

 

  • Boxes outline clear, step-by-step procedures, and provide a handy reference for future lab work
  • Tables emphasize practical information for comparison and contrast and some include additional tips and guidance.
  • The Glossary lists important terminology found in each of the nine units of the book. 
  • Worked-through Example Problems appear in nearly every chapter. They:
    • Help students apply new information and many provide insight into laboratory calculations (particularly useful for students who find math intimidating)
    • Include answers to all practice problems 
    • Provide discussion questions based on laboratory “scenarios” that may be used for student writing assignments and discussion 
    • Increase the relevance of the information for beginning students, using illustrations, case studies, anecdotes, and familiar examples
    • Highlight the importance of the work of the bench scientist
  • Fundamental laboratory skills that are seldom explicitly described in biology lab manuals are included and they emphasize:
    • Preparing reagents for biological systems
    • How to document procedures in a laboratory notebook  
    • Safety practices
    • How to use common laboratory instruments  
  • Issues relating to the commercial biotechnology workplace include regulatory affairs, product life cycles, and documentation in order to
    • Make students aware of many career opportunities in biotechnology
    • Introduce them to biotechnology products, the life cycles of products, and the role of the biotechnology laboratory   
  • The Biotechnology Project Website provides teacher and student resources including PowerPoint® presentations, a laboratory manual, and examples of activities that instructors might find useful.(www.matcmadison.edu/biotech/resources).

    • Unit 1 (Chapters 1-3) presents a stronger introduction to the nature of the biotechnology workplace.
      • Chapter 1 is updated to include recent discoveries in biotechnology. 
      • Chapter 2 features new case studies, a new section on intellectual property, and more information about the biotechnology industry workplace and available  career opportunities.
      • NEW! Chapter 3, “Pharmaceutical/Biopharmaceutical Products,” is entirely new and discusses the role of the laboratory in the biotechnology industry.
    • Revised and updated quality unit (Unit 2) includes new safety regulations that reflect the increased need for safety in dealing with biologically hazardous materials.
    • An updated unit on quality assays and tests (Unit 6) provides current information on equipment and methods.
    • A new chapter on preparing culture media (Chapter 30) has been added.
    • The computer unit (Unit 9) has been completely rewritten to keep pace with rapidly advancing technologies.

 

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I. INTRODUCTION TO THE BIOTECHNOLOGY WORK PLACE

1. The Modern Biotechnology Industry: A Broad Overview

2. The Business of Biotechnology: The Transformation of Knowledge into Products

3. Pharmaceutical/Biopharmaceutical Products

 

II. PRODUCT QUALITY AND BIOTECHNOLOGY

4. Introduction to Product Quality Systems

5. Biotechnology and the Regulation of Food and Medical Products

6. Documentation, the Foundation of Quality

7. Quality Systems in the Production Facility

8. Quality Systems in the Laboratory

 

III. SAFETY IN THE LABORATORY

9. Introduction to a Safe Workplace

10. Working Safely in the Laboratory: General Considerations and Physical Hazards

11. Working Safely with Chemicals

12. Working Safely with Biological Materials

 

IV. MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW

13. Basic Math Techniques

14. Proportional Relationships

15. Relationships and Graphing

16. Descriptions of Data (Descriptive Statistics)

 

V. LABORATORY MEASUREMENTS

17. Introduction to Quality Laboratory Measurements, Tests and Assays

18. Introduction to Instrumental Methods and Electricity

19. The Measurement of Weight

20. The Measurement of Volume

21. The Measurement of Temperature

22. The Measurement of pH, Selected Ions and Conductivity

23. Measurements Involving Light A. Basic Principles and Instrumentation

 

VI. QUALITY ASSAYS AND TESTS

24. Introduction to Quality Laboratory Tests and Assays

25. Measurements Involving Light B. Applications and Methods

 

VII. LABORATORY SOLUTIONS 

26. Preparation of Laboratory Solutions A: Concentration Expressions and Calculations

27. Preparation of Laboratory Solutions B. Basic Procedures and Practical Information

28. Solutions: Associated Procedures and Information

29. Laboratory Solutions to Support the Activity of Biological Macromolecules

30. Culture Media for Intact Cells

 

VII. BASIC SEPARATION METHODS

30. Introduction to Filtration

31. Introduction to Centrifugation

32. Introduction to Bioseparations

 

IX. COMPUTERS IN THE LABORATORY

33. Computers: An Overview

34: Data Handling with Computers

35. Applications of the Internet to Biotechnology

    A primary text for courses that focus on basic laboratory methods in biotechnology, and an ancillary text for any biology course that includes a laboratory component that want to focus on biotechnology.

    Unique in both content and approach, this text/reference provides a systematic introduction to basic laboratory methods from the perspective of the everyday work in the labs of the biotechnology/bioscience industry. Written at a level that is accessible to beginning college students with average math and science backgrounds. Provides the depth and detail useful to professional laboratory workers. It blends the background and theory provided by introductory chemistry texts with the practical concerns discussed in advanced lab manuals. It focuses on basic laboratory methods, the principles that underlie those methods, and the equipment that makes laboratory work possible.

  • 0137955359Basic Laboratory Methods for Biotechnology
    Seidman & Moore
    © 2000 | Benjamin Cummings | Spiral Bound; 751 pages | Instock
    ISBN-10: 0137955359 | ISBN-13: 9780137955350
    Brief Description

Lisa Seidman received her Ph.D. from the University of Wisconsin and has taught for more than 20 years in the Biotechnology Laboratory Technician Program at Madison Area Technical College in Madison, Wisconsin. Cynthia Moore received her Ph.D. in Microbiology from Temple University School of Medicine. She is currently an Associate Professor of Biological Sciences at Illinois State University, where she also serves as Director of Biology Teacher Education.

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